Part:BBa_K2538006:Design

Microglia specific promoter pF4/80

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Designed using Geneious to meet registry standards. Part was cloned into pSB1C3 using EcoRI and PstI restriction sites.

Source

Murine genomic DNA sequence. Based on O'Reilly, D., Addley, M., Quinn, C., MacFarlane, A. J., Gordon, S., McKnight, A. J., & Greaves, D. R. (2004). Functional analysis of the murine Emr1 promoter identifies a novel purine-rich regulatory motif required for high-level gene expression in macrophages. Genomics, 84(6), 1030-1040.‏ https://www.ncbi.nlm.nih.gov/pubmed/15533720/

References

Rosario, A. M., Cruz, P. E., Ceballos-Diaz, C., Strickland, M. R., Siemienski, Z., Pardo, M., ... & Golde, T. E. (2016). Microglia-specific targeting by novel capsid-modified AAV6 vectors. Molecular Therapy-Methods & Clinical Development, 3.‏